Part:BBa_K4384006

5YSS_WT

This cyclase is expected to convert the molecule 5AVA to valerolactam. This cyclase is modified by our team by mutating the 152th residue tyrosine to glutamic acid, and the 184th residue tryptophan to phenylalanine. We have modified the cyclase as it was reported that Y152E has the high specific activity of 28.5 ± 1.3 U/mg of detecting ω-amino fatty acids, such as 5-AVA and 6-ACA, via lactam formation. This cyclase is found present in Citrobacter freundii. [1]

References: [1] Yeom, S.-J.; Kim, M.; Kwon, K. K.; Fu, Y.; Rha, E.; Park, S.-H.; Lee, H.; Kim, H.; Lee, D.-H.; Kim, D.-M.; Lee, S.-G. A Synthetic Microbial Biosensor for High-Throughput Screening of Lactam Biocatalysts. Nature Communications 2018, 9 (1). https://doi.org/10.1038/s41467-018-07488-0.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 10
  
• 1000
  COMPATIBLE WITH RFC[1000]